BPA toxicity during development of zebrafish embryo

Abstract Bisphenol A (BPA) is a monomer used in the production of polycarbonate, polymer commonly found plastics, epoxy resins and thermal papers. The presence BPA food, water, air dust has been great concern recent years not only due to environmental ecological issues but also because its supposed risk public health related mutagenic carcinogenic potential. In this study we evaluated toxicity bisphenol zebrafish embryos (Danio rerio) determined 50% lethal concentration (LC50) chemical. was at concentrations ranging from 1 μM 100 E3 medium/0.5% dimethylsulfoxide (DMSO) previously prepared stock solutions 100% DMSO. Controls included exposed medium or supplemented with 0.5% Camptothecin (CPT), known inhibitor cell proliferation as positive control 0.001 Adults were placed for breeding day before experimental set up, then, viable collected selected use. Experiments carried out triplicates, according specifications Organization Economic Cooperation Development (OECD). One embryo/well (25 per concentration) distributed 96 well microplates absence chemicals. plates kept BOD incubators controlled temperature 28.5 ºC photoperiod 14 h light:10 dark. After 24h, 48h, 72h 96h exposure, following parameters: mortality, coagulation, rate heartbeat, hatching morphological abnormalities. Photography obtained by photomicroscopy. Apoptosis DNA ladder assay. extracted phenol:chloroform method analyzed 2% agarose gel electrophoresis. fragments visualized after ethidium bromide staining ultraviolet transilluminator. LC50 70 24 hours, 72 48 47 hours 31 exposure. induced physiological alterations such yolk sac pericardial edema, delay inhibition, spine deformation, decreasing heartbeat mortality. conclusion, demonstrated that marked malformations above 25 corroborating current concerns widespread air, food water humans bodily fluids tissues.